Isolation and Characterization of the Total DNA for Staphylococcus aureus Isolated from Surgical Wound Infections, Non Patients Nares, and Urinary Tract Infections

A total of 60 samples were isolated from surgical wound infections, nares, and UTI's.
Eight samples were positive for Staphylococcus aureus biochemical tests. These samples
were collected from the Dr. Khalid General hospital in Koya city.
Analysis of plasmids that digested by EcoRI and Hind III showed that plasmids
conferring different resistance phenotypes showed different restriction profiles, while
plasmids conferring similar resistance phenotypes may have shared one or more bands in
common.
Generally, Staphylococcus aureus isolates were highly diverse, as shown by their
differing chromosome and plasmid patterns, and antibiotic resistance profiles. Plasmids
isolated from surgical wound infections showed similarity with plasmids isolated from
urinary tract infections depending on the antibiotic resistance and deportation gel
electrophoresis. There was no similarity between the plasmids isolated from isolates of non
pathogenic and pathogenic state.