Treatment of Murine Mammary Adenocarcinoma Cell Line (AMN3) With Polyvinylpyrrolidone Coated Silver Nanoparticles

In this research, we have synthesized silver nanoparticles with a pulsed laser using a metallic silver (purity; 99.99%), the targeted metallic silver piece was submerged in a liquid containing Polyvinylpyrrolidone (PVP). The produced nanoparticles were observed with different methods included UV-Vis Spectrometer, Atomic Absorption (AAS) and Electron Microscope (TEM). All peak absorption spectra for the two types of nanoparticles were ranged between 401 and 411 nm whereas nanoparticles size were in the range between 1 and 70 nm. The malignant anti-proliferation activity of nanoparticles was tested on murine mammary gland cancer cell line (AMN3). This activity was with potent inhibitory effect on the cellular proliferation rate of the cells under investigation. The inhibitory action was elevated when both the concentration of silver nanoparticles (AgNPs) and time of exposure were increased in comparison with the control untreated cells. Staining the cells with crystal violet stain revealed an increase in the level of cellular destruction. The results demonstrated that exposing the malignant cells to the nanomaterial of silver increases the proportion of the cells that undergo mitochondrial membrane disruption and DNA fragmentation and that will result in the induction of apoptosis. These results confirm that PVP-coated AgNPs induces apoptosis and cancer cell death.