Role of CD200 and CD43 in Diagnosis and Prognosis of CLL and NHL Patients

Background: Chronic lymphoproliferative disorder
(LPD), is a malignant disease of lymphocytes in the blood and
lymphatic tissue. Chronic lymphocytic leukaemia, it is the
commonest type of chronic lymphoproliferative disorder.
Scoring by immunophenotyping is used to differentiate B-cell
chronic lymphocytic leukemia from other B-Non-Hodgkin
lymphomas. CD200 (OX2) is a glycoprotein of membrane, it is
related to type I superfamily of immunoglobulin .
CD43 (Sialophorin) is a sialoglycoprotein that is present on the
surface of T lymphocytes, some B lymphocytes, granulocytes and
monocytes, that play important role for immune function and also
play a role as physiologic ligand-receptor complex involved in T
cell activation.
Aim of study-Evaluate the role of CD200 and CD43 positive
expression and co-expression in diagnosis and prognosis of CLL
and NHL.
Subjects and Methods: This cross sectional study on one hundred
forty five patients with chronic lymphoprliferative disorders who
were attending Baghdad teaching hospital at medical city from
beginning of January 2020 to end of December 2020, patients
divided in to two groups; chronic lymphocytic leukemia (CLL)
and non-Hodgkin lymphoma (NHL) patients At diagnosis, there
was assessment of CD200 and CD43. Clinical and laboratory data
were done including staging by modified Rai, and Ann arbor
staging system ( for CLL and NHL respectively), then follow up
for about 6-12.
Results: There is significant statistical correlation between CD 200
and CD 43 and often co-expression of both in differentiation
between CLL and NHL (p value < 0.001).
Almost all patients of CD 200 positive expression show moderate
to bright pattern of expression in CLL in apposite to NHL patients,
who showed dim to moderate pattern of expression, while the great
majority of CD43 expression was dim to moderate pattern in both
CLL and NHL patients.In CLL patients there was no significant
correlation between prognostic markers (age, Hb, platelets count,
lymphocytes count and CD 38 expression) and CD200, CD 43.
For NHL patients, all markers show no significant correlation
except that CD 43 show significant correlation with expression of
CD 38.
Conclusion: CD200, CD43 and often co-expression of both have a
significant value in diagnosis and differentiation of CLL from B
NHLs.