Evolution Mitochondrial tRNAtrp Encoded Gene Variation in Colorectal Cancer

Background: Colorectal cancer (CRC) is a significant disease in Iraq that increased in the last years, the role of Transfer RNA (tRNA) types in cell functions still under investigations in different disease, tRNA level, genetic variation, and its derivative molecules were studied. Objective: the present study aims to evaluate mitochondrial tRNAtrp encoded gene variation in colorectal cancer in a case control design. Methodology: about 48 CRC patients with 30 healthy individuals were enrolled in the present study, PCR –sequencing method was used to read the whole tRNAtrp gene sequence, variations were detection according to NCBI SNPs. Results: The results showed that the patient group has two types of CRC, adenocarcinoma was high frequent (80%) than Mucinus adenocarcinoma (20%), four stages; the III stage was more observed than others (54.28%), and according to the grade, the well differentiation was more seen (57.14%) than moderate and poor differentiation. The genetic analysis showed a significant association of tRNAtrp total genetic variation with CRC (p <0.0014), about 15 SNPs were detected in the patients group and 1 SNP in the control group, non-significant association of SNPs with CRC. Conclusion: according to present findings, the variation of tRNAtrp encoded may be associated with CRC.