Smail, Shukur Wasman and Abdulqadir, Shang Ziyad and Alalem, Lana Sardar Saleh and Rasheed, Taban Kamal and Khudhur, Zhikal Omar and Mzury, Abdullah Faqiyazdin Ahmed and Awla, Harem Khdir and Ghayour, Mohammad B. and Abdolmaleki, Arash (2024) Enhancing Sciatic Nerve Regeneration with Osteopontin-Loaded Acellular Nerve Allografts in Rats: Effects on Macrophage Polarization. Tissue and Cell, 88. p. 102379. ISSN 00408166
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Abstract
Osteopontin (OPN) is a multifunctional matrix glycoprotein with neuroprotective and immunomodulatory properties. This study explored the potential of OPN-loaded acellular nerve allografts (ANAs) to repair sciatic nerves in male Wistar rats. The research also delved into the impact of OPN on macrophage phenotypes. We reconstructed a 10 mm nerve gap with ANAs containing OPN at 2 nM and 4 nM. The sciatic functional index (SFI) and paw withdrawal reflex latency (WRL) showed the significant efficacy of ANA/OPN (2 nM) in enhancement of target organ reinnervation and subsequent sensorimotor recovery compared to other groups. Electrophysiological and histomorphometric analyses further supported the regenerative properties of ANA/OPN (2 nM). Additionally, ANA/OPN (2 nM) promoted macrophage polarization towards an M2 phenotype and reduced proinflammatory cytokines at the injury site. In conclusion, the study suggested that ANA loaded with 2 nM OPN effectively repaired transected sciatic nerves in rats, potentially through enhancing axonal sprouting and exerting anti-inflammatory effects.
Item Type: | Article |
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Uncontrolled Keywords: | Osteopontin (OPN), Acellular Nerve Allografts (ANAs), Sciatic Nerve Repair, Macrophage Polarization, M2 Phenotype |
Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
Divisions: | Department of Medical Microbiology > Research papers |
Depositing User: | ePrints Depositor |
Date Deposited: | 20 Nov 2024 14:17 |
Last Modified: | 20 Nov 2024 14:17 |
URI: | https://eprints.cihanuniversity.edu.iq/id/eprint/2898 |