The Combined Supplementary Impact of Ashwagandha, Curcumin, and Green Tea on Hematological Alterations in Benzene-Induced Leukemia in Albino Rats

Objective: Leukemia is characterized by the proliferation of immature white blood cells caused by different factors. Benzene is a chemical solution that can cause leukemia development when exposed to it in a given duration and quantity. Methodology: In this study, pure benzene was used to induce leukemia in adult male albino rats by intravenous injection of chromasolve benzene solution for 3 weeks every 2 days. Following leukemia induction, rats were divided into 4 groups (6 rats each), and orally treated by gavage with (200mg/kg, 250mg/kg, /200mg/kg) of (ashwagandha, green tea, curcumin) as a combination group which was administrated for daily for 1 Month. Another treatment group was given (300mg/kg) of ashwagandha as a treatment after the treatment period (28 days) rats were cervically dislocated after blood samples were taken by drawing blood directly from the heart. Hematological and histological parameters of (bone marrow- spleen- liver) were carried and evaluated after dissection. Results: Both treatments increased total WBC counts compared to the negative control group. Although the combination treatment resulted in a slightly higher WBC count, the difference was minor, showing that Ashwagandha alone is also effective in enhancing immune activity. Likewise, RBC counts improved in both treatment groups compared to the control negative group, additionally, both treatments effectively increased red blood cell levels. According to the previous studies resent of Blast cells indicating for leukemic activity. All treatment groups were shown normal levels of blast cell counts. Combination group—which exhibited the lowest blast cell counts, indicating that combination group was more effective than the ashwagandha-treated group. Histological studies of bone marrow, spleen, and liver have shown tissue structure preserved in the treated group comparing with benzene induced leukemia group. However, the group receiving Ashwagandha supplement shown better tissue structure compared to the combination-treated group. Conclusion: In summary, ashwagandha has strong anti-leukemic and protective properties against benzene-induced toxicity both individually and in conjunction with curcumin and green tea. Its potential as a synergistic treatment option is suggested by the combination therapy's enhanced therapeutic advantages, which include decreased monocyte levels, increased lymphocyte levels, and greater histological protection in bone marrow, liver and spleen organs.