Preparation and Evaluation of Local Medium for Culture and Growth of Trichomonas vaginalis Isolated From Patients in Mosul, Iraq

This study was conducted to prepare a local culture medium from orange leaves for cultivation of Trichomonas vaginalis. Five culture media, namely: Oxide Trichomonas media (CM 161), CPLM, Barbarowski, Protose pepton and Pavlova, which are used for clinical diagnosis of vaginal trichomoniasis were compared in vitro to this new medium. Growth studies using fresh clinical isolates from different patients showed clear ability of Trichomonas to grow on this medium. The organisms inoculated initially (25´l04 cm-3) into this media
reached a population of (78´l04 cm3) organism in 72 hours. Although (CM 161) and (CPLM) media allows more prolific growth over a shorter period of time (24 hours). It was obvious that the orange leaves media (OLM) was suitable for
detecting T. vaginalis in patients with vaginitis.