Hybridoma Technique in Pharmaceutical Science

Hybridomas are the specific cells that have specific character to produce desire antibody. To produce
monoclonal antibodies, β cells are removed from the spleen of an animal that has been immunized with the releant
antigen. These β cells are then fused with myeloma tumor cells in the presence of PEG. The use of HGPRT cells
assured that only hybridomas are selected. The fused hybrid cells (hybridomas), produce large amounts of the desired
antibodies. They have to be selected and cloned. Cloning is done after identification of positive primary hybridoma cells. The hybridoma technique to produce monoclonal antibody was first invented by Cesar Milstein, Niels Kaj Jerne and Georges J. F. Köhler in 1975.