Identification Biofilm Producers Staphylococcus aureus Isolates and Detect their Biofilm Genes from Gingivitis Cases

Gingivitis is one of the most common oral disorders and is caused by the accumulation of plaque in people with poor oral hygiene. The purpose of this study is to detect Staphylococcus aureus biofilm producers in cases of gingivitis and detect their biofilm genes. During the months of November 2018 and February 2019, a sample of fifty patients diagnosed clinically with gingivitis were collected from the laboratory of the Faculty of Dentistry at Hawler Medical University. Using the traditional culture method and vitek 2, eleven S. aureus bacteria isolates were identified. Using the Congo red agar method, biofilm detection was performed to test the ability to form biofilm. The S. aureus isolates were put through a series of tests to see if the genes responsible for the biofilm could be found (PCR). Biofilm formation method of detection using Congo red agar showed that 10 (90.1%) of S. aureus were biofilm positive and one 1 (9.1%) were biofilm negative. As well as the results of molecular analysis by using PCR showed that isolated S. aureus were carried biofilm genes icaC (100%), icaD (100%), cna (90.9%) and fnba (100%). Gingivitis is one of the most common oral diseases in our area, and this research found that the majority of S. aureus isolates carry the icaC, icaD, cna, and fnba Biofilm genes.