The Impact of Grape Juice Extract on Liver Structure of Castrated Rats

The effects of ingestion of freshly prepared ethanolic
extracts of 6g/kg/day of black grape juice was investigated on some
biochemical, parameters and histological structure of liver in
castrated male rats for 42 days. Twenty-one male rats (Rattus
norvegicus) were used in the present study. Rats are divided into
three groups of animals (N= 7). Tap water was given to the intact
and castrated rats in groups 1 and 2. Daily, 1 ml (6g/kg/day) of
grape extract was administered intragastrically to the castrated
rats in group three. The biochemical test for several parameters
did not reveal any differences between the groups that were being
compared. Whereas the values of HDL showed a significant
difference in both castrated and castrated treated subjects as
compared with control animals (P<0.01; P<0.05) respectively, as
well as the results of VLDL showed a significant difference in
castrated treated group (P< 0.02). However, the value of ALP also
showed a significant difference in castrated treated group
(P<0.03). Castration had a major impact on the histological
structure of the liver, resulting in lobular inflammation,
ballooning degeneration, and macrovesicular steatoses. The liver
structure of castrated rats was found to be improved effectively by
grape juice treatment, as indicated by histological sections
showing a moderate normal architecture to some extent.
Administration of grape extract was significantly affecting the
histological regeneration of the liver in castrated rats.
Keywords: liver; castration; grape juice; central vein.