An Investigation of the Variances (rs5744168, rs5744174) of TLR-5 and Susceptibility to Polycystic Ovary Syndrome and Toxoplasmosis in Groups of Iraqi Women

Polycystic Ovary Syndrome (PCOS) is a prevalent endocrine disorder characterized by a range of reproductive, metabolic, and immune irregularities. Emerging evidence suggests that infectious agents can contribute to the pathogenesis of PCOS, particularly through immune dysregulation. Toxoplasma gondii, an intracellular protozoan parasite, has been implicated in various immune-mediated disorders. Toll-like Receptor 5 (TLR-5), a key component of the innate immune system, plays a pivotal role in recognizing pathogen-associated molecular patterns, including those from T. gondii. This article explores interplay between TLR- 5 single nucleotide polymorphisms (rs5744168, rs5744174) and susceptibility to T. gondii and the potential implications for PCOS development. A case- control study of 150 sample patient divided into four groups, amplification-refractory mutation system (ARMS PCR) was used to examine these SNPs. rs5744168 shows a significant difference between PCOS+ toxoplasmosis and toxoplasmosis only with control group. Also, rs5744174 revealed a significant difference between toxoplasmosis patients and control group. Therefore, women that have both SNPs (rs5744168, rs5744174) in TLR-5 become more susceptible to infection with toxoplasma gondii.