Vibrio Cholera 22: Isolation And Molecular Detection of Cholera Toxin (CTX) in Some Iraqi Governorates

Vibrio cholerae is a major etiology of diarrhea in humans worldwide. In this study, we isolated and identified V. cholerae from the human stool of suspected cases admitted in different hospitals of Iraqi governerate. A total of (180) suspected patient stool samples infected with Vibreo cholera (V. cholera) ,110 sample were positive and showed growth of yellow colored colonies when cultured on Thiosulfate citrate bile salt agar(TCBS ). The isolates were Gram-negative, curved shaped, and motile. Biochemically, they were found positive for indole and Methyl Red tests and negative for Voges– Proskauer test when we used API 20 test. Ninty of the isolates were ogawa when made serotyping test. Antibiotic sensitivity test revealed these isolates as highly sensitive to chloramphenicol, tetracycline m Ampicillinm , and Erythromycin while resistant to TR , and oxacillin. After the routine culture of samples for isolation and identification of V. cholerae isolates, PCR was performed for molecular detection of V. cholerae isolates based on 16S ribosomal RNA and ompW genes. Toxigenicity was detected by CTX toxin genes. Out of the 110 positive samples, only 50 isolates were positive for CTX gene, using genus-specific primers.