Molecular Identification of a Newly Isolated of Niallia Circulans from Different Wounds in Erbil City/Iraq

Molecular identification using 16S rRNA (PCR), sequencing, and the GenBank database, of newly isolated N. circulans, from gunshot wound among clinical wounds in several hospitals in Erbil, Iraq, and studying the antibiogram profile of isolates were the objectives of this study. A total of 157 clinical specimens from various wound infections were collected during (June 2022-September 2022). Molecular identification16S rRNA, morphological characterization and biochemical behavior identified a total of 60 (38.21%) Gram positive bacteria were detected, of which one (1.6%) N. circulans was newly reported in Iraq. The application of PCR in the field of genetic diagnostics has grown to the point where it is now acknowledged as the norm approach for identifying nucleic acids from variety number of sample and microbial types. The percentages of resistance among 20 different antimicrobials showed by N. circulans strain was 100% resist to 11 antibiotics Amoxicillin-Clavulanic acid, Ampicillin Chloramphenicol, Cefotaxime, Nalidixic acid, Nitrofurantoin, Norfloxacin, Rifampin, Streptomycin, Tetracycline and Trimethoprim. Antibiotic-resistant strains of the opportunistic human pathogens Niallia circulans (previously known as Bacillus circulans) have been isolated, raising concerns about the rising prevalence of antibiotic resistance in pathogenic bacteria globally. In conclusion, gunshot wound is a potential source of infection caused by newly isolated of N. circulans, treatment of nosocomial infections should be guided by antibiotic susceptibility testing.