Spectrophotometric Determination of Escitalopram Oxalate in Pure Form and its Pharmaceutical Preparation via Oxidative Coupling Reaction

background: One of the techniques for spectroscopic determination are the oxidative coupling reaction, have been utilized in the estimation of compounds in many fields, such as agriculture, clinical, and pharmacological analysis. Aims: The aim of this studying to locate a sensitive and cheap technique for the estimation of escitalopram-oxalate (ESC) in pharmacological compositions and in pure form. Methods and Material: The process relies on the coupling between the escitalopram oxalate with 4-aminoantipyrine (4-AAP) in a basic medium in the attend of N-Bromosuccinimide (NBS) to produce an intense red azo dye, soluble in water with max. Abs. at 528 nm against the blank solution. Statistical analysis used: The UV–Vis spectra were exported to MS Excel software. All statistics were analyzed by using SPASS Program Version 18. Results: The method has obeyed Beer's law in the concentration range of (1.0-20) μg/ml with a relative error of 0.16-1.30 and a relative standard deviation of 0.852-2.936 depending on the concentration level. The values of molar absorptivity(ε), Sandell's sensitivity, and limit of detection were 1.4628x104 L mole-1 cm-1 , 0.0283μg/cm2, and 0.1061μg/ml, respectively. Conclusions The method used to synthesize azo dye is an easy, fast, and inexpensive method and gives high sensitivity and absorbency, and the wavelength of 528 is where the maximum absorption occurs. It was also discovered that the dye's color stays stable at room temperature.