Determination of Antibacterial Activities of Some Salicylaldimine Derived-Schiffbase with their Copper Complexes on Some Pathogenic Bacteria

The identification of novel potent chemicals against drug-resistant bacteria is critical to combat the alarming issue of microbial resistance toward antibiotics. There is a high risk of resistant infections spreading uncontrollably due to a lack of new and efficient antibiotics. So regarding
to this manner, we tested the antibacterial activity of some salicylaldimine-derived Schiff bases with their Copper (Cu) complexes and Gentamicin (Genta) as four different concentration 1 µM, 10 µM, 100 µM and 1000 µM, by our newly modified MTT assay protocol against three bacterial strains of Streptococcus pyogenes(S. pyogenes), Klebsiella pneumonia(K. pneumonia), and Proteus mirabilis(P.mirabilis) which they have high risk for drug resistance development
and analyzed their Optical density(OD) data in order to get MIC50 values. And as a result,the lowest and most effective MIC50 value of Genta was 1.6µM for P. mirabilis followed by 1.8 and 2.4 µM for S. pyogenes and K. pneumonia respectively. The compound ligands and their complexes had effect differently on these bacteria based on their different cellular structure and some of the complexes showed better antibacterial activity than their original ligands, regarding with observation of unique bacterial response toward our MTT protocol.