Immunohistohemical and Molecular Studies of Mouse Liver in Castrated Subjects treated with Grape Juice

The present study was designed to evaluate the alterations in the liver tissues especially Ito and Kupff er cells of both castrated and castrated mice treated with10 μl/g of grape juice. The present study was conducted on forty fi ve healthy males of Swiss albino mice, which were divided into 3 groups (N= 15 mice per each group). The fi rst group was intact (control); the second one was castrated and the third group was castrated treated with 10 μl/g of grape juice. The immunohistochemical and molecular sections of liver tissue from castrated group stained with vimentin antibody, showed a strong intensity of positive stain (+++), as compared with sections from intact group (control) which showed a weak intensity of positive stain (+). On the other hand sections from castrated subjects treated with 10μl/g grape juice revealed a moderate intensity of positive stain(++) as compared with control group. The present study concluded that surgical castration aggravated increased hepatic steatosis and increased infl ammatory response by increased activation of Kupff er and Ito cells, these eff ects could be reserved by aggravated increased grape juice administration.